02363nas a2200445 4500008004100000022001400041245007900055210006900134260001600203300001200219490000800231520109900239653001201338653001501350653002501365653002101390653001801411653001601429653001101445653001701456653002201473653001101495653002501506653001301531653001901544653001301563653002901576653000901605653001301614653002501627653003001652653002501682653001501707653002601722100002701748700002501775700001801800700004001818856005901858 2003 eng d a0027-842400aImmunomicelles: targeted pharmaceutical carriers for poorly soluble drugs.0 aImmunomicelles targeted pharmaceutical carriers for poorly solub c2003 May 13 a6039-440 v1003 a
To prepare immunomicelles, new targeted carriers for poorly soluble pharmaceuticals, a procedure has been developed to chemically attach mAbs to reactive groups incorporated into the corona of polymeric micelles made of polyethylene glycol-phosphatidylethanolamine conjugates. Micelle-attached antibodies retained their ability to specifically interact with their antigens. Immunomicelles with attached antitumor mAb 2C5 effectively recognized and bound various cancer cells in vitro and showed an increased accumulation in experimental tumors in mice when compared with nontargeted micelles. Intravenous administration of tumor-specific 2C5 immunomicelles loaded with a sparingly soluble anticancer agent, taxol, into experimental mice bearing Lewis lung carcinoma resulted in an increased accumulation of taxol in the tumor compared with free taxol or taxol in nontargeted micelles and in enhanced tumor growth inhibition. This family of pharmaceutical carriers can be used for the solubilization and enhanced delivery of poorly soluble drugs to various pathological sites in the body.
10aAnimals10aAntibodies10aBiological Transport10aBreast Neoplasms10aDrug Carriers10aDrug Design10aFemale10aFluoresceins10aFreeze Fracturing10aHumans10aIndium Radioisotopes10aKinetics10aLung Neoplasms10aLymphoma10aMetabolic Clearance Rate10aMice10aMicelles10aMicroscopy, Electron10aPhosphatidylethanolamines10aPolyethylene Glycols10aSolubility10aTumor Cells, Cultured1 aTorchilin, Vladimir, P1 aLukyanov, Anatoly, N1 aGao, Zhonggao1 aPapahadjopoulos-Sternberg, Brigitte uhttps://www.microfluidicsciences.com/drupal/?q=node/24