03473nas a2200553 4500008004100000022001400041245012800055210006900183260001300252300001100265490000700276520186800283653001002151653002202161653003902183653001702222653001002239653001102249653002802260653001102288653002402299653001602323653002902339653003002368653002302398653001802421653003102439100002102470700002702491700001702518700001302535700002302548700002002571700001902591700001902610700001802629700002102647700002402668700002202692700002702714700001602741700002302757700001502780700001502795700001602810700001402826700001902840856006002859 2011 eng d a1526-097600aAssociation of elevated E6 oncoprotein with grade of cervical neoplasia using PDZ interaction-mediated precipitation of E6.0 aAssociation of elevated E6 oncoprotein with grade of cervical ne c2011 Apr a169-760 v153 a
OBJECTIVE: To determine the expression of human papillomavirus (HPV) type 16 E6 oncoprotein in cervical specimens of women with and without cervical intraepithelial neoplasia (CIN).
MATERIALS AND METHODS: Cervical specimens from 2,530 unscreened women aged 30 to 54 years from Shanxi, China, were obtained. All women were assessed by liquid-based cytology, high-risk HPV DNA tests, and colposcopy with directed biopsy and endocervical curettage as necessary. Women with abnormal cytologic results or positive HPV DNA results were recalled for colposcopy, 4-quadrant cervical biopsies, and endocervical curettage. Women with biopsy-proven CIN and cancer and a convenience sample of HC2-positive, disease-negative women were tested for the presence of HPV-16 infection via HPV-16 E6 DNA-specific polymerase chain reaction. A PDZ interaction-mediated E6 oncoprotein precipitation method followed by E6-specific Western blot was performed on specimens from women with HPV-16 infections. Associations between elevated expression of E6 oncoprotein and CIN 2 and 3 were determined using logistic regression and a reference category of CIN 1 and disease-negative.
RESULTS: A significant trend for the detection of HPV-16 E6 oncoprotein in specimen of women with proven HPV-16 infection was determined: 0% (0/12), 12.5% (1/8), 36.4% (4/11), and 42.9% (3/7) of those with negative findings, CIN 1, 2, and 3, respectively (p = .01). Compared with the category combining negative findings and CIN 1, detection of E6 oncoprotein was associated with CIN 2 (odds ratio = 10.9, p = .05) and CIN 3 (odds ratio = 14.3, p = .04).
CONCLUSIONS: There is a significant association between elevated expression of E6 oncoprotein and grade of CIN. This finding seems consistent with the role played by E6 oncoprotein in carcinogenesis.
10aAdult10aBlotting, Western10aCervical Intraepithelial Neoplasia10aCervix Uteri10aChina10aFemale10aHuman papillomavirus 1610aHumans10aImmunoprecipitation10aMiddle Aged10aOncogene Proteins, Viral10aPapillomavirus Infections10aRepressor Proteins10aUp-Regulation10aUterine Cervical Neoplasms1 aSellors, John, W1 aSchweizer, Johannes, G1 aLu, Peter, S1 aLiu, Bin1 aWeigl, Bernhard, H1 aCui, Jian, Feng1 aPeck, Roger, B1 aLewis, Kristen1 aLim, Jeanette1 aHoward, Michelle1 aMahoney, Charles, W1 aMcAllister, Linda1 aBerard-Bergery, Marthe1 aBry, Claire1 aLabiad, Yassine, A1 aLi, Haimin1 aLiu, Lilyn1 aSilver, Jon1 aChen, Wen1 aQiao, You, Lin uhttps://www.microfluidicsciences.com/drupal/?q=node/211