02409nas a2200277   4500008004100000022001400041245012900055210006900184260000900253300001100262490000900273520153100282653001901813653001201832653001101844653002401855653003601879653004201915100001801957700001701975700001901992700001902011700002102030700002002051856006002071       2010                        eng d  a1557-170X00aNon-instrumented nucleic acid amplification (NINA): instrument-free molecular malaria diagnostics for low-resource settings.0 aNoninstrumented nucleic acid amplification NINA instrumentfree m  c2010  a1097-90 v20103 a<p>We have achieved the first complete, non-instrumented nucleic acid amplification test (NAAT) using a calcium oxide heat source thermally linked to an engineered phase change material. These two components alone maintain a thermal profile suitable for the loop-mediated isothermal amplification assay. Starting with computational fluid dynamics analysis, we identified nominal geometry for the exothermic reaction chamber, phase change material chamber, thermal insulation, and packaging. Using this model, we designed and fabricated an alpha prototype assay platform. We have verified the function of this multi-pathogen-capable platform with both fluorescent and visual turbidity indications using samples spiked with malaria DNA. Both the exothermically heated platform samples and samples heated on a Perkin-Elmer GeneAmp9600 thermocycler were first incubated at 62°C for 45 minutes, then heated to 95°C to terminate enzyme activity, then analyzed. Results from the exothermically heated, non-instrumented platform were comparable to those from the thermocycler. These developments will enable point-of-care diagnostics using accurate NAATs which until now have required a well-equipped laboratory. The aim of this research is to provide pathogen detection with NAAT-level sensitivity in low-resource settings where assays such as immunochromatographic strip tests are successfully used but where there is no access to the infrastructure and logistics required to operate and maintain instrument-based diagnostics.</p>10aDNA, Protozoan10aHeating10aHumans10aMalaria, Falciparum10aMolecular Diagnostic Techniques10aNucleic Acid Amplification Techniques1 aLaBarre, Paul1 aGerlach, Jay1 aWilmoth, Jared1 aBeddoe, Andrew1 aSingleton, Jered1 aWeigl, Bernhard  uhttps://www.microfluidicsciences.com/drupal/?q=node/215