01618nas a2200169   4500008004100000245018300041210006900224260000800293520077700301100002401078700001901102700002401121700002401145700002701169700002101196856023101217       2008                        eng d00aUnited {States} {Patent}: 7416892 - {Method} and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing0 aUnited States Patent 7416892 Method and system for microfluidic   caug3 aA microfluidic system for isolation and amplification of DNA or RNA from aqueous solutions and detection of the DNA or RNA on a lateral flow detection strip, including a disposable microfluidic card for use in analysis of bacteria in platelets and an analysis of sexually transmitted diseases (STD) in urine. The card will include an embedded membrane that filters out cells and cellular debris. Any biological debris on the membrane will be lysed and the DNA or RNA amplified via PCR amplification protocol, including appropriate reagents and thermal cycling conditions. The amplified DNA or RNA are transferred to a lateral flow detection strip for a visual diagnostic read out. An alternate embodiment includes a microfluidic card for use in typing antiglobulin assays.1 aBattrell, Frederick1 aShen, Mingchao1 aWeigl, Bernhard, H.1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBreidford, Wayne  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=13&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%22