01142nas a2200169   4500008004100000245009900041210006900140260000800209520038500217100002200602700001900624700002400643700002700667700002400694700002400718856023000742       2014                        eng d00aUnited {States} {Patent}: 8697009 - {Microfluidic} devices for fluid manipulation and analysis0 aUnited States Patent 8697009 Microfluidic devices for fluid mani  capr3 aThe present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.1 aSaltsman, Patrick1 aShen, Mingchao1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBattrell, Frederick1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=3&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201142nas a2200169   4500008004100000245009900041210006900140260000800209520038500217100002200602700001900624700002400643700002700667700002400694700002400718856023000742       2013                        eng d00aUnited {States} {Patent}: 8557198 - {Microfluidic} devices for fluid manipulation and analysis0 aUnited States Patent 8557198 Microfluidic devices for fluid mani  coct3 aThe present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.1 aSaltsman, Patrick1 aShen, Mingchao1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBattrell, Frederick1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=4&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201142nas a2200169   4500008004100000245009900041210006900140260000800209520038500217100002200602700001900624700002400643700002700667700002400694700002400718856023000742       2012                        eng d00aUnited {States} {Patent}: 8318109 - {Microfluidic} devices for fluid manipulation and analysis0 aUnited States Patent 8318109 Microfluidic devices for fluid mani  cnov3 aThe present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.1 aSaltsman, Patrick1 aShen, Mingchao1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBattrell, Frederick1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=6&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2202044nas a2200169   4500008004100000245012100041210006900162260000800231520126200239100002501501700002701526700002101553700002401574700002201598700002401620856023001644       2010                        eng d00aUnited {States} {Patent}: 7648835 - {System} and method for heating, cooling and heat cycling on microfluidic device0 aUnited States Patent 7648835 System and method for heating cooli  cjan3 aAn integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.1 aBreidford, Wayne, L.1 aLancaster, Christy, A.1 aHayenga, Jon, W.1 aBardell, Ronald, L.1 aTonn, Jeffrey, F.1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=9&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2202046nas a2200169   4500008004100000245012100041210006900162260000800231520126200239100002101501700002701522700002601549700002401575700002201599700002401621856023101645       2009                        eng d00aUnited {States} {Patent}: 7544506 - {System} and method for heating, cooling and heat cycling on microfluidic device0 aUnited States Patent 7544506 System and method for heating cooli  cjun3 aAn integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.1 aBreidford, Wayne1 aLancaster, Christy, A.1 aHayenga, Jon, Wallace1 aBardell, Ronald, L.1 aTonn, Jeffrey, F.1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=11&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201618nas a2200169   4500008004100000245018300041210006900224260000800293520077700301100002401078700001901102700002401121700002401145700002701169700002101196856023101217       2008                        eng d00aUnited {States} {Patent}: 7416892 - {Method} and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing0 aUnited States Patent 7416892 Method and system for microfluidic   caug3 aA microfluidic system for isolation and amplification of DNA or RNA from aqueous solutions and detection of the DNA or RNA on a lateral flow detection strip, including a disposable microfluidic card for use in analysis of bacteria in platelets and an analysis of sexually transmitted diseases (STD) in urine. The card will include an embedded membrane that filters out cells and cellular debris. Any biological debris on the membrane will be lysed and the DNA or RNA amplified via PCR amplification protocol, including appropriate reagents and thermal cycling conditions. The amplified DNA or RNA are transferred to a lateral flow detection strip for a visual diagnostic read out. An alternate embodiment includes a microfluidic card for use in typing antiglobulin assays.1 aBattrell, Frederick1 aShen, Mingchao1 aWeigl, Bernhard, H.1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBreidford, Wayne  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=13&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201143nas a2200169   4500008004100000245009900041210006900140260000800209520038500217100002200602700001900624700002400643700002700667700002400694700002400718856023100742       2008                        eng d00aUnited {States} {Patent}: 7419638 - {Microfluidic} devices for fluid manipulation and analysis0 aUnited States Patent 7419638 Microfluidic devices for fluid mani  csep3 aThe present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.1 aSaltsman, Patrick1 aShen, Mingchao1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBattrell, Frederick1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=12&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201727nas a2200145   4500008004100000245007800041210006900119260000800188520105700196100002101253700002401274700002401298700002801322856023101350       2007                        eng d00aUnited {States} {Patent}: 7223371 - {Microfluidic} channel network device0 aUnited States Patent 7223371 Microfluidic channel network device  cmay3 aDescribed herein is microfluidic device for joining fluids and a related method for doing the same. The device according to the present invention includes a microfluidic junction, an outlet channel, and a plurality of circuit units. A microfluidic junction is an area for converging multiple fluids. An outlet channel is capable of receiving fluid from the microfluidic junction. An outlet channel includes a first end connected with the microfluidic junction, a second end connected with a waste reservoir, and an analysis region positioned between the first end and the second end of the outlet channel. The device also includes a plurality of circuit units. Each circuit unit includes a source channel with a first end capable of receiving sample fluid and a second end connected with the microfluidic junction; a branch channel connected with the source channel at an intersection; and a flow diversion system capable of differentially directing fluid flowing through a source channel either into the microfluidic junction or into a branch channel.1 aHayenga, Jon, W.1 aWeigl, Bernhard, H.1 aBardell, Ronald, L.1 aMorris, Christopher, J.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=15&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201902nas a2200133   4500008004100000245007500041210006600116520127800182100002401460700001601484700002001500700001701520856023101537       2007                        eng d00aUnited {States} {Patent}: 7271007 - {Microscale} diffusion immunoassay0 aUnited States Patent 7271007 Microscale diffusion immunoassay3 aMethods and apparatuses are provided for determining presence and concentration of analytes by exploiting molecular binding reactions and differential diffusion rates. Analyte particles and binding particles are allowed to diffuse toward each other, and slowing of the diffusion front is detected when they meet. From the position of the diffusion front, presence and concentration of analyte particles can be determined. One embodiment provides a competitive immunoassay in a microfluidic format. This diffusion immunoassay (DIA) relies on measuring the concentration of labeled antigen along one dimension of a microchannel after allowing it to diffuse for a short time into a region containing specific antibodies. A simple microfluidic device, the T-Sensor, was used to implement a DIA to measure the concentration of phenytoin, a small drug molecule. Concentrations of analyte over the range of 50 to 1600 nM can be measured in less than a minute. The assay is homogeneous, rapid, requires only microliter volumes of reagents and sample, and is applicable to a wide range of analytes, including therapeutic drugs, molecular biological markers, and environmental contaminants. Methods for separating particles of similar size in a diffusion separator are also provided.1 aWeigl, Bernhard, H.1 aYager, Paul1 aKamholz, Andrew1 aHatch, Anson  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=14&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201426nas a2200169   4500008004100000245014600041210006900187260000800256520063200264100002400896700002400920700002000944700002000964700002000984700002101004856023101025       2006                        eng d00aUnited {States} {Patent}: 7011791 - {Microfluidic} devices for rotational manipulation of the fluidic interface between multiple flow streams0 aUnited States Patent 7011791 Microfluidic devices for rotational  cmar3 aMicrofluidic devices and methods are provided for enhancing detection of a diffusion pattern formed by particles diffusing between at least two fluid streams in parallel laminar flow such that an interface is formed between them by increasing the dimension of the streams in the diffusion direction. This may be accomplished by flowing the streams through a transforming turn, or by flowing the streams through a channel having diverging walls. Devices and methods are also provided for enhancing diffusion between two streams comprising changing the interface between said streams from a narrow interface to a broad interface.1 aWeigl, Bernhard, H.1 aBardell, Ronald, L.1 aKamholz, Andrew1 aMunson, Matthew1 aSchilling, Eric1 aHawkins, Kenneth  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=17&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2200850nas a2200121   4500008004100000245006700041210005800108520026300166100002000429700002400449700002400473856023100497       2004                        eng d00aUnited {States} {Patent}: 6674525 - {Split} focusing cytometer0 aUnited States Patent 6674525 Split focusing cytometer3 aA microcytometer which combines lysing and cytometry into a unified system that achieves blood lysis and white blood cell count in a single device. The device focuses the white cells into a thin ribbon which is then focused into a single stream for analysis.1 aBardell, Ronald1 aWeigl, Bernhard, H.1 aBattrell, Frederick  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=20&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201427nas a2200145   4500008004100000245006700041210005800108260000800166520079500174100002000969700002400989700001801013700001901031856023101050       2004                        eng d00aUnited {States} {Patent}: 6742661 - {Well}-plate microfluidics0 aUnited States Patent 6742661 Well plate microfluidics  cjun3 aMicrofluidic devices and methods for performing a microfluidic process are presented. A microfluidic device conforms with a standard well plate format. The device includes a well plate comprising a plate and an array of wells formed on or in the plate, and a microfluidic structure connecting at least two of the wells. The device can rely exclusively on gravitational and capillary forces that exist in channels within the microfluidic structure when receiving fluid streams. Also disclosed is a microfluidic device having an array of microfluidic structures, each connecting at least two wells of a well plate, and connecting three or more wells in alternative embodiments. With the present invention, a large number of microfluidic processes or reactions can be performed simultaneously.1 aSchulte, Thomas1 aWeigl, Bernhard, H.1 aMorris, Chris1 aKesler, Natasa  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=19&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201057nas a2200157   4500008004100000245006500041210005600106260000800162520037900170100002400549700002600573700002100599700002400620700002400644856023100668       2004                        eng d00aUnited {States} {Patent}: 6743399 - {Pumpless} microfluidics0 aUnited States Patent 6743399 Pumpless microfluidics  cjun3 aA microfluidic device which operates without the need for an external power source. The device includes a body structure, at least one microscale channel within the structure, a port for introducing fluid into the channel, and a power source internal to the structure for propelling the fluid through the channel. Various structures are described which embody the invention.1 aWeigl, Bernhard, H.1 aWilliams, Clinton, L.1 aHayenga, Jon, W.1 aBardell, Ronald, L.1 aSchulte, Thomas, E.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=18&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201922nas a2200145   4500008004100000245007500041210006600116260000800182520127800190100002401468700001601492700002001508700001701528856023101545       2003                        eng d00aUnited {States} {Patent}: 6541213 - {Microscale} diffusion immunoassay0 aUnited States Patent 6541213 Microscale diffusion immunoassay  capr3 aMethods and apparatuses are provided for determining presence and concentration of analytes by exploiting molecular binding reactions and differential diffusion rates. Analyte particles and binding particles are allowed to diffuse toward each other, and slowing of the diffusion front is detected when they meet. From the position of the diffusion front, presence and concentration of analyte particles can be determined. One embodiment provides a competitive immunoassay in a microfluidic format. This diffusion immunoassay (DIA) relies on measuring the concentration of labeled antigen along one dimension of a microchannel after allowing it to diffuse for a short time into a region containing specific antibodies. A simple microfluidic device, the T-Sensor, was used to implement a DIA to measure the concentration of phenytoin, a small drug molecule. Concentrations of analyte over the range of 50 to 1600 nM can be measured in less than a minute. The assay is homogeneous, rapid, requires only microliter volumes of reagents and sample, and is applicable to a wide range of analytes, including therapeutic drugs, molecular biological markers, and environmental contaminants. Methods for separating particles of similar size in a diffusion separator are also provided.1 aWeigl, Bernhard, H.1 aYager, Paul1 aKamholz, Andrew1 aHatch, Anson  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=23&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2200985nas a2200145   4500008004100000245010200041210006900143260000800212520029400220100002400514700002200538700002400560700002400584856023100608       2003                        eng d00aUnited {States} {Patent}: 6557427 - {Capillaries} for fluid movement within microfluidic channels0 aUnited States Patent 6557427 Capillaries for fluid movement with  cmay3 aA capillary for introduction of whole blood into an analysis device. The capillary has a variable volume along its length, which allows the liquid sample to be drawn into the interior of the cartridge, away from the inlet, reducing the risk of contamination of the sample from the outside.1 aWeigl, Bernhard, H.1 aKlein, Gerald, L.1 aBardell, Ronald, L.1 aBattrell, Frederick  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=22&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201710nas a2200145   4500008004100000245013300041210006900174520099400243100002401237700001901261700001801280700002001298700001501318856023101333       2003                        eng d00aUnited {States} {Patent}: 6582963 - {Simultaneous} analyte determination and reference balancing in reference {T}-sensor devices0 aUnited States Patent 6582963 Simultaneous analyte determination 3 aA reference T-sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlets in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of analyte particles by a detectable change in property when contacted with the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or an internal standard stream; c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area; and (d) an outlet for conducting the streams out of the laminar flow channel preferably to form a single mixed stream.1 aWeigl, Bernhard, H.1 aHoll, Mark, R.1 aZebert, Diane1 aKenny, Margaret1 aWu, Caicai  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=21&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201023nas a2200133   4500008004100000245009300041210006900134260000800203520039800211100002400609700002000633700000500653856023100658       2002                        eng d00aUnited {States} {Patent}: 6409832 - {Protein} crystallization in microfluidic structures0 aUnited States Patent 6409832 Protein crystallization in microflu  cjun3 aA device for promoting protein crystal growth (PCG) using microfluidic channels. A protein sample and a solvent solution are combined within a microfluidic channel having laminar flow characteristics which forms diffusion zones, providing for a well defined crystallization. Protein crystals can then be harvested from the device. The device is particularly suited for microgravity conditions.1 aWeigl, Bernhard, H.1 aSygusch, Jurgen1 a  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=26&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201903nas a2200253   4500008004100000245007800041210006900119260000800188520096500196100002401161700001601185700002101201700001901222700002201241700002001263700002301283700002001306700001901326700002001345700001801365700002001383700001501403856023101418       2002                        eng d00aUnited {States} {Patent}: 6454945 - {Microfabricated} devices and methods0 aUnited States Patent 6454945 Microfabricated devices and methods  csep3 aThis invention provides microfabricated systems for extraction of desired particles from a sample stream containing desired and undesired particles. The sample stream is placed in laminar flow contact with an extraction stream under conditions in which inertial effects are negligible. The contact between the two streams is maintained for a sufficient period of time to allow differential transport of the desired particles from the sample stream into the extraction stream. In a preferred embodiment the differential transport mechanism is diffusion. The extraction system of this invention coupled to a microfabricated diffusion-based mixing device and/or sensing means allows picoliter quantities of fluid to be processed or analyzed on devices no larger than silicon wafers. Such diffusion-based mixing or sensing devices are preferably channel cell systems for detecting the presence and/or measuring the quantity of analyte particles in a sample stream.1 aWeigl, Bernhard, H.1 aYager, Paul1 aBrody, James, P.1 aHoll, Mark, R.1 aForster, Fred, K.1 aAltendorf, Eric1 aGalambos, Paul, C.1 aKenny, Margaret1 aSchutte, David1 aHixson, Gregory1 aZebert, Diane1 aKamholz, Andrew1 aWu, Caicai  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=25&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2200967nas a2200145   4500008004100000245007400041210006500115520029000180100002400470700002200494700002400516700002600540700002400566856023100590       2002                        eng d00aUnited {States} {Patent}: 6488896 - {Microfluidic} analysis cartridge0 aUnited States Patent 6488896 Microfluidic analysis cartridge3 aA device for analyzing sample solutions such as whole blood based on coagulation and agglutination which requires no external power source or moving parts to perform the analysis. Single disposable cartridges for performing blood typing assays can be constructed using this technology.1 aWeigl, Bernhard, H.1 aKlein, Gerald, L.1 aBardell, Ronald, L.1 aWilliams, Clinton, L.1 aSchulte, Thomas, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=24&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2202375nas a2200157   4500008004100000245013300041210006900174260000800243520163900251100002401890700001901914700001801933700002001951700001501971856023101986       2001                        eng d00aUnited {States} {Patent}: 6171865 - {Simultaneous} analyte determination and reference balancing in reference {T}-sensor devices0 aUnited States Patent 6171865 Simultaneous analyte determination   cjan3 aA reference sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlet means in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or internal standard stream; and, c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of said streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area. Branching channels may be provided as outlet means for conducting the streams out of the laminar flow channel. Also provided are methods of using the device to improve accuracy and increase convenience of measuring the concentration of analytes in a sample. Monitoring reference stream(s) in laminar flow with sample stream(s) allows for calibration or for monitoring or correcting for the effects of experimental conditions which might otherwise degrade accuracy. A reference stream which serves as both a control stream and an internal standard stream is convenient and economical. The device of this invention also needs less frequent calibration than previously-known fluid analysis devices because the devices and methods of this invention provide for continuous and simultaneous quality control and internal standardization.1 aWeigl, Bernhard, H.1 aHoll, Mark, R.1 aZebert, Diane1 aKenny, Margaret1 aWu, Caicai  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=30&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201095nas a2200109   4500008004100000245013500041210006900176520046500245100002400710700002000734856023100754       2000                        eng d00aUnited {States} {Patent}: 6091502 - {Device} and method for performing spectral measurements in flow cells with spatial resolution0 aUnited States Patent 6091502 Device and method for performing sp3 aA device and method for performing spectral measurements in flow cells with spatial resolution using a variable transmission optical filter having at least two areas with different optical properties. Light from a light source passes through the variable transmission filter to a flow cell containing a sample to be analyzed. The resultant light pattern is sensed by a detecting means, which analyzes the spectral properties of the sample within the flow cell.1 aWeigl, Bernhard, H.1 aAltendorf, Eric  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=34&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201074nas a2200145   4500008004100000245010600041210006900147260000800216520038500224100002200609700002300631700002400654700001900678856023100697       2000                        eng d00aUnited {States} {Patent}: 6134950 - {Method} for determining concentration of a laminar sample stream0 aUnited States Patent 6134950 Method for determining concentratio  coct3 aA method and apparatus including programmed computers are provided for determining the viscosity of a first stream in a laminar flow and a second stream in a laminar flow, the flow rates, the centerline of the flow channel, and the position of the interface between the streams with respect to the centerline, and for calculating viscosity ratio of the first stream to the second.1 aForster, Fred, K.1 aGalambos, Paul, C.1 aWeigl, Bernhard, H.1 aHoll, Mark, R.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=33&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2202381nas a2200157   4500008004100000245013300041210006900174260000800243520164500251100002401896700001901920700001801939700002001957700001501977856023101992       1999                        eng d00aUnited {States} {Patent}: 5948684 - {Simultaneous} analyte determination and reference balancing in reference {T}-sensor devices0 aUnited States Patent 5948684 Simultaneous analyte determination   csep3 aA reference T-sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlet means in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or internal standard stream; c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area; and d) outlet means for conducting the streams out of the laminar flow channel preferably to form a single mixed stream. Also provided are methods of using the device to improve accuracy and increase convenience of measuring the concentration of analytes in a sample. Monitoring reference stream(s) in laminar flow with sample stream(s) allows for calibration or for monitoring or correcting for the effects of experimental conditions which might otherwise degrade accuracy. A reference stream which serves as both a control stream and an internal standard stream is convenient and economical. The device of this invention also needs less frequent calibration than previously-known fluid analysis devices because the devices and methods of this invention provide for continuous and simultaneous quality control and internal standardization.1 aWeigl, Bernhard, H.1 aHoll, Mark, R.1 aZebert, Diane1 aKenny, Margaret1 aWu, Caicai  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=37&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201808nas a2200229   4500008004100000245009000041210006900131260000800200520092700208100002401135700001601159700002101175700001901196700002001215700001901235700002001254700001801274700002001292700001501312700002001327856023101347       1999                        eng d00aUnited {States} {Patent}: 5972710 - {Microfabricated} diffusion-based chemical sensor0 aUnited States Patent 5972710 Microfabricated diffusionbased chem  coct3 aA channel-cell system is provided for detecting the presence and/or measuring the presence of analyte particles in a sample stream comprising: a) a laminar flow channel; b) two inlets in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may comprise an indicator substance which indicates the presence of the analyte particles by a detectable change in property when contacted with the analyte particles, and (2) the sample stream; c) wherein the laminar flow channel has a depth sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to the substantial exclusion of the larger particles in the sample stream to form a detection area; and d) an outlet for conducting the streams out of the laminar flow channel to form a single mixed stream.1 aWeigl, Bernhard, H.1 aYager, Paul1 aBrody, James, P.1 aHoll, Mark, R.1 aKenny, Margaret1 aSchutte, David1 aHixson, Gregory1 aZebert, Diane1 aKamholz, Andrew1 aWu, Caicai1 aAltendorf, Eric  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=36&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201104nas a2200133   4500008004100000245010600041210006900147520043500216100002200651700002300673700002400696700001900720856023100739       1999                        eng d00aUnited {States} {Patent}: 5974867 - {Method} for determining concentration of a laminar sample stream0 aUnited States Patent 5974867 Method for determining concentratio3 aMethods and apparatuses including programmed computers are provided for determining the initial concentration of diffusible particles in a sample stream introduced into a system wherein the sample stream which contains the diffusible particles flows in adjacent laminar flow with an indicator stream containing an indicator substance capable of exhibiting an observable change at a known concentration of the diffusible particles.1 aForster, Fred, K.1 aGalambos, Paul, C.1 aWeigl, Bernhard, H.1 aHoll, Mark, R.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=35&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201613nas a2200145   4500008004100000245009000041210006900131260000800200520094800208100001601156700002401172700002101196700001901217856023101236       1998                        eng d00aUnited {States} {Patent}: 5716852 - {Microfabricated} diffusion-based chemical sensor0 aUnited States Patent 5716852 Microfabricated diffusionbased chem  cfeb3 aA channel-cell system is provided for detecting the presence and/or measuring the presence of analyte particles in a sample stream comprising: a) a laminar flow channel; b) two inlet means in fluid connection with said laminar flow channel for respectively conducting into said laminar flow channel (1) an indicator stream which may comprise an indicator substance which indicates the presence of said analyte particles by a detectable change in property when contacted with said analyte particles, and (2) said sample stream; c) wherein said laminar flow channel has a depth sufficiently small to allow laminar flow of said streams and a length sufficient to allow particles of said analyte to diffuse into said indicator stream to the substantial exclusion of said larger particles in said sample stream to form a detection area; and d) outlet means for conducting said streams out of said laminar flow channel to form a single mixed stream.1 aYager, Paul1 aWeigl, Bernhard, H.1 aBrody, James, P.1 aHoll, Mark, R.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=39&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201512nas a2200109   4500008004100000245008800041210006900129520092700198100002201125700002401147856023101171       1998                        eng d00aUnited {States} {Patent}: 5747349 - {Fluorescent} reporter beads for fluid analysis0 aUnited States Patent 5747349 Fluorescent reporter beads for flui3 aThe present invention provides a method and apparatus for rapid measurement of a fluid bulk analyte, requiring only microscale volumes. Several fluid bulk analytes can be measured simultaneously and, for biological samples, the cell content can also be measured simultaneously. The invention comprises reporter beads for chemical analysis of fluid bulk properties such as pH, oxygen saturation and ion content. Each reporter bead comprises a substrate bead having a plurality of at least one type of fluorescent reporter molecules immobilized thereon. The fluorescent properties of the reporter bead are sensitive to a corresponding analyte. Reporter beads are added to a fluid sample and the analyte concentration is determined by measuring fluorescence of individual beads, for example in a flow cytometer. Alternatively, reporter molecules which change absorbance as a function of analyte concentration can be employed.1 avan den Engh, Ger1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=38&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%22