@article {211, title = {Association of elevated E6 oncoprotein with grade of cervical neoplasia using PDZ interaction-mediated precipitation of E6.}, journal = {J Low Genit Tract Dis}, volume = {15}, year = {2011}, month = {2011 Apr}, pages = {169-76}, abstract = {

OBJECTIVE: To determine the expression of human papillomavirus (HPV) type 16 E6 oncoprotein in cervical specimens of women with and without cervical intraepithelial neoplasia (CIN).

MATERIALS AND METHODS: Cervical specimens from 2,530 unscreened women aged 30 to 54 years from Shanxi, China, were obtained. All women were assessed by liquid-based cytology, high-risk HPV DNA tests, and colposcopy with directed biopsy and endocervical curettage as necessary. Women with abnormal cytologic results or positive HPV DNA results were recalled for colposcopy, 4-quadrant cervical biopsies, and endocervical curettage. Women with biopsy-proven CIN and cancer and a convenience sample of HC2-positive, disease-negative women were tested for the presence of HPV-16 infection via HPV-16 E6 DNA-specific polymerase chain reaction. A PDZ interaction-mediated E6 oncoprotein precipitation method followed by E6-specific Western blot was performed on specimens from women with HPV-16 infections. Associations between elevated expression of E6 oncoprotein and CIN 2 and 3 were determined using logistic regression and a reference category of CIN 1 and disease-negative.

RESULTS: A significant trend for the detection of HPV-16 E6 oncoprotein in specimen of women with proven HPV-16 infection was determined: 0\% (0/12), 12.5\% (1/8), 36.4\% (4/11), and 42.9\% (3/7) of those with negative findings, CIN 1, 2, and 3, respectively (p = .01). Compared with the category combining negative findings and CIN 1, detection of E6 oncoprotein was associated with CIN 2 (odds ratio = 10.9, p = .05) and CIN 3 (odds ratio = 14.3, p = .04).

CONCLUSIONS: There is a significant association between elevated expression of E6 oncoprotein and grade of CIN. This finding seems consistent with the role played by E6 oncoprotein in carcinogenesis.

}, keywords = {Adult, Blotting, Western, Cervical Intraepithelial Neoplasia, Cervix Uteri, China, Female, Human papillomavirus 16, Humans, Immunoprecipitation, Middle Aged, Oncogene Proteins, Viral, Papillomavirus Infections, Repressor Proteins, Up-Regulation, Uterine Cervical Neoplasms}, issn = {1526-0976}, doi = {10.1097/LGT.0b013e3181f6c84d}, author = {Sellors, John W and Schweizer, Johannes G and Lu, Peter S and Liu, Bin and Weigl, Bernhard H and Cui, Jian Feng and Peck, Roger B and Lewis, Kristen and Lim, Jeanette and Howard, Michelle and Mahoney, Charles W and McAllister, Linda and Berard-Bergery, Marthe and Bry, Claire and Labiad, Yassine A and Li, Haimin and Liu, Lilyn and Silver, Jon and Chen, Wen and Qiao, You Lin} } @article {221, title = {A magnetic immunochromatographic strip test for detection of human papillomavirus 16 E6.}, journal = {Clin Chem}, volume = {52}, year = {2006}, month = {2006 Nov}, pages = {2170-2}, keywords = {Antibodies, Monoclonal, Chromatography, Enzyme-Linked Immunosorbent Assay, Female, Human papillomavirus 16, Humans, Magnetics, Oncogene Proteins, Viral, PDZ Domains, Protein Binding, Protein Interaction Mapping, Recombinant Fusion Proteins, Repressor Proteins, Sensitivity and Specificity}, issn = {0009-9147}, author = {Peck, Roger B and Schweizer, Johannes and Weigl, Bernhard H and Somoza, Chamorro and Silver, John and Sellors, John W and Lu, Peter S} }