TY - JOUR T1 - Association of elevated E6 oncoprotein with grade of cervical neoplasia using PDZ interaction-mediated precipitation of E6. JF - J Low Genit Tract Dis Y1 - 2011 A1 - Sellors, John W A1 - Schweizer, Johannes G A1 - Lu, Peter S A1 - Liu, Bin A1 - Weigl, Bernhard H A1 - Cui, Jian Feng A1 - Peck, Roger B A1 - Lewis, Kristen A1 - Lim, Jeanette A1 - Howard, Michelle A1 - Mahoney, Charles W A1 - McAllister, Linda A1 - Berard-Bergery, Marthe A1 - Bry, Claire A1 - Labiad, Yassine A A1 - Li, Haimin A1 - Liu, Lilyn A1 - Silver, Jon A1 - Chen, Wen A1 - Qiao, You Lin KW - Adult KW - Blotting, Western KW - Cervical Intraepithelial Neoplasia KW - Cervix Uteri KW - China KW - Female KW - Human papillomavirus 16 KW - Humans KW - Immunoprecipitation KW - Middle Aged KW - Oncogene Proteins, Viral KW - Papillomavirus Infections KW - Repressor Proteins KW - Up-Regulation KW - Uterine Cervical Neoplasms AB -

OBJECTIVE: To determine the expression of human papillomavirus (HPV) type 16 E6 oncoprotein in cervical specimens of women with and without cervical intraepithelial neoplasia (CIN).

MATERIALS AND METHODS: Cervical specimens from 2,530 unscreened women aged 30 to 54 years from Shanxi, China, were obtained. All women were assessed by liquid-based cytology, high-risk HPV DNA tests, and colposcopy with directed biopsy and endocervical curettage as necessary. Women with abnormal cytologic results or positive HPV DNA results were recalled for colposcopy, 4-quadrant cervical biopsies, and endocervical curettage. Women with biopsy-proven CIN and cancer and a convenience sample of HC2-positive, disease-negative women were tested for the presence of HPV-16 infection via HPV-16 E6 DNA-specific polymerase chain reaction. A PDZ interaction-mediated E6 oncoprotein precipitation method followed by E6-specific Western blot was performed on specimens from women with HPV-16 infections. Associations between elevated expression of E6 oncoprotein and CIN 2 and 3 were determined using logistic regression and a reference category of CIN 1 and disease-negative.

RESULTS: A significant trend for the detection of HPV-16 E6 oncoprotein in specimen of women with proven HPV-16 infection was determined: 0% (0/12), 12.5% (1/8), 36.4% (4/11), and 42.9% (3/7) of those with negative findings, CIN 1, 2, and 3, respectively (p = .01). Compared with the category combining negative findings and CIN 1, detection of E6 oncoprotein was associated with CIN 2 (odds ratio = 10.9, p = .05) and CIN 3 (odds ratio = 14.3, p = .04).

CONCLUSIONS: There is a significant association between elevated expression of E6 oncoprotein and grade of CIN. This finding seems consistent with the role played by E6 oncoprotein in carcinogenesis.

VL - 15 IS - 2 U1 - http://www.ncbi.nlm.nih.gov/pubmed/21478701?dopt=Abstract ER -