01695nas a2200253 4500008004100000022001400041245007100055210006900126260001600195300001100211490000700222520098300229653001901212653003101231653001101262100001601273700001501289700001601304700001501320700001301335700001501348700001901363856005901382 1994 eng d a0168-165600aOptical triple sensor for measuring pH, oxygen and carbon dioxide.0 aOptical triple sensor for measuring pH oxygen and carbon dioxide c1994 Feb 14 a127-380 v323 a
A triple sensor unit consisting of opto-chemical sensors for measurement of pH, oxygen and carbon dioxide in bioreactors is presented. The pH and the CO2 sensor are based on the color change of a pH-sensitive dye immobilized on a polymeric support. The resulting changes in absorption are monitored through optical fibers. The oxygen sensor is based on the quenching of the fluorescence of a metal-organic dye. All three sensors are fully LED compatible. The sensitive membranes consist of plastic films and can be stored and replaced conveniently. The sensors are sterilizable with hydrogen peroxide and ethanol. In addition, the pH sensor is steam sterilizable. Accuracy, resolution and reproducibility fulfill the requirements for use in biotechnological applications. Calibration procedures for each sensor are presented. The working principle and the performance of all three sensors are described, with particular emphasis given to their application in bioreactors.
10aCarbon Dioxide10aHydrogen-Ion Concentration10aOxygen1 aWeigl, B, H1 aHolobar, A1 aTrettnak, W1 aKlimant, I1 aKraus, H1 aO'Leary, P1 aWolfbeis, O, S uhttp://www.microfluidicsciences.com/drupal/?q=node/232