01751nas a2200217   4500008004100000022001400041245009200055210006900147260001500216300001100231490000700242520109300249653001301342653002101355653003101376100001801407700001601425700002001441700001301461856005901474       1999                        eng d  a0003-270000aQuantitative analysis of molecular interaction in a microfluidic channel: the T-sensor.0 aQuantitative analysis of molecular interaction in a microfluidic  c1999 Dec 1  a5340-70 v713 a<p>The T-sensor is a recently developed microfluidic chemical measurement device that exploits the low Reynolds number flow conditions in microfabricated channels. The interdiffusion and resulting chemical interaction of components from two or more input fluid streams can be monitored optically, allowing measurement of analyte concentrations on a continuous basis. In a simple form of T-sensor, the concentration of a target analyte is determined by measuring fluorescence intensity in a region where the analyte and a fluorescent indicator have interdiffused. An analytical model has been developed that predicts device behavior from the diffusion coefficients of the analyte, indicator, and analyte--indicator complex and from the kinetics of the complex formation. Diffusion coefficients depend on the local viscosity which, in turn, depends on local concentrations of all analytes. These relationships, as well as reaction equilibria, are often unknown. A rapid method for determining these unknown parameters by interpreting T-sensor experiments through the model is presented.</p>10aKinetics10aModels, Chemical10aSpectrometry, Fluorescence1 aKamholz, A, E1 aWeigl, B, H1 aFinlayson, B, A1 aYager, P  uhttp://www.microfluidicsciences.com/drupal/?q=node/229